- Tutor is available to students
High Performance Liquid Chromatography
Classroom based course with study materials, lecturer support and CPD Hours included
London Training For Excellence
Summary
Overview
Description
- Inroduction to analytical approaches
- Operating principles for major analytical methods
- Introduction to adsorption, absorption, liquid chromatography
- Separation columns
- LC vs HPLC
- Configuration of HPLC
- Adsorbent [solid phase] attributes and functionality
- Solvents [pressurized liquid phase] attributes and functionality
- Investigation of solid – fluid phase interactions and optimization
- Sizing of HPLC
- Comparison to other analytical methods; advantages and shortcomings
- Examples and case studies
- Isocratic elution and gradient elution modes
- Partition chromatography
- Displacement chromatography
- Reverse phase chromatography
- Normal phase chromatography
- Size exclusion chromatographyIon exchange chromatography
- Aqueous normal phase chromatography
- Advances and current status
- Review of applications
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