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High Performance Liquid Chromatography
Classroom based course with study materials, lecturer support and CPD Hours included
London Training For Excellence
Summary
Overview
Analytical methods play a vital role in any laboratory and any industrial process. They are used as a means of identifying concentrations and compositions of different phases and mixes under varying conditions and accuracy requirements. Besides the traditional analytical methods, other versions of them are currently popular and widespread, such as High performance liquid chromatography [HPLC]. HPLC offers superb advantages in comparison to classical liquid chromatography as discussed in this scientific course. Modes of operation, principles, installation are also described in detail.
Variations of the suggested outlines can be prepared on demand (terms and conditions apply)
Description
- Inroduction to analytical approaches
- Operating principles for major analytical methods
- Introduction to adsorption, absorption, liquid chromatography
- Separation columns
- LC vs HPLC
- Configuration of HPLC
- Adsorbent [solid phase] attributes and functionality
- Solvents [pressurized liquid phase] attributes and functionality
- Investigation of solid – fluid phase interactions and optimization
- Sizing of HPLC
- Comparison to other analytical methods; advantages and shortcomings
- Examples and case studies
- Isocratic elution and gradient elution modes
- Partition chromatography
- Displacement chromatography
- Reverse phase chromatography
- Normal phase chromatography
- Size exclusion chromatographyIon exchange chromatography
- Aqueous normal phase chromatography
- Advances and current status
- Review of applications
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