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High Performance Liquid Chromatography

Classroom based course with study materials, lecturer support and CPD Hours included


London Training For Excellence

Summary

Price
£3,500 inc VAT
Study method
Online
Duration
5 hours · Self-paced
Qualification
No formal qualification
Additional info
  • Tutor is available to students

Overview

Analytical methods play a vital role in any laboratory and any industrial process. They are used as a means of identifying concentrations and compositions of different phases and mixes under varying conditions and accuracy requirements. Besides the traditional analytical methods, other versions of them are currently popular and widespread, such as High performance liquid chromatography [HPLC]. HPLC offers superb advantages in comparison to classical liquid chromatography as discussed in this scientific course. Modes of operation, principles, installation are also described in detail.

Variations of the suggested outlines can be prepared on demand (terms and conditions apply)

Description

  • Inroduction to analytical approaches
  • Operating principles for major analytical methods
  • Introduction to adsorption, absorption, liquid chromatography
  • Separation columns
  • LC vs HPLC
  • Configuration of HPLC
  • Adsorbent [solid phase] attributes and functionality
  • Solvents [pressurized liquid phase] attributes and functionality
  • Investigation of solid – fluid phase interactions and optimization
  • Sizing of HPLC
  • Comparison to other analytical methods; advantages and shortcomings
  • Examples and case studies
  • Isocratic elution and gradient elution modes
  • Partition chromatography
  • Displacement chromatography
  • Reverse phase chromatography
  • Normal phase chromatography
  • Size exclusion chromatographyIon exchange chromatography
  • Aqueous normal phase chromatography
  • Advances and current status
  • Review of applications

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