Research Scientist - Molecular Biology
Job Type: Initially a 3 month to 6 month contract
Working hours: Full time, Monday to Friday 9am to 5pm
Note: UK and EU Nationals (and those with a Valid Visa to work in the UK)
The Client is a world famous Research Institute for biomedical research into human health and disease. Their Biomedical Research Facility contains a Genetic Manipulation service providing resources, specialist skills, production, storage and use of genetically modified animal and cell lines.
You would be a part of the GMS team whose services include:
· Micromanipulation - introduction of gene sequence by pronuclear or cytoplasmic microinjection one cell embryos, ES cell microinjection or blastocysts or 8 cell embryos and aggregation.
· Surgical expertise such as embryo transfer and specialism in other surgical techniques and training in aseptic techniques
· Cryopreservation of rodent and aquatic germplasm for archiving and backup of GA lines
· Rederivation of live and frozen imports & exports to and from the Institute
· Molecular biology -design and production of sequences for targeting strategies, with a focus on CRISPR
· Microbiome monitoring through 16s Sequencing
· Genetic Stability Monitoring of inbred
· Genotyping of commonly used GA lines
· Mouse Stem Cell culture, maintenance and transfection, derivation and characterisation
You will increase and then maintain the throughput of embryo microinjection that is related to the creation of genetically modified models from design steps to quality control checks on the resulting models. Crispr cas based gene editing is a key technology in use and you will have scope to gain in depth experience in the delivery of this by cytoplasmic and pronuclear techniques as well as through ES cell injection. In addition embryo transfer experience would be valued.
In your role, your objectives will include, but not be limited to;
· Microinjection of zygotes through cytoplasmic and pronuclear route
· Microinjection of blastocyst and/ or 8 cell embryos with embryonic stem cells to produce chimeric embryos and animals
· To use the available databases to keep good and accurate records of ongoing work and to communicate effectively with other team members and service users.
· To perform embryo transfer surgery to the institute requirements and standards.
Health & Safety - You will be:
· aware of all relevant & safety Guidance and legislation
· ensure all staff are comply with SOP's
· responsible for reviewing and updating SOP's, COSHH and RA's to share with staff as appropriate
IT - You will also
· order consumables via the system, ensuring value for money and maintain the budget for the service
· use the colony management and other appropriate storage and procedural databases
Communication - in your role you will also need the use your ability to:
· interpret and communicate information effectively to staff across all levels
· liaise effectively and efficiently to ensure that projects are completed both efficiently and in a timely manner
· build and maintain relationships with all stakeholders
· chair and participate in committees and working groups
· provide written and verbal reports including those related to animal welfare, production, scientific procedures and health and safety matters.
The essential skills and experience you should possess are:
· Qualification in a biological subject, in animal technology or a related science (Several years' experience in a relevant subject may be acceptable instead)
· The post holder will possess experience and have a proven track record gained in a previous molecular biology position
· Experience of contributing effectively to the work of a team, a flexible and cooperative approach to working with others
· Experience with or willingness to work on animal models
· Able to work independently and drive a project forward.
· Good project planning, time-keeping, organisational and record-keeping skills
If you have these skills and or experience it will be to your advantage:
§ Degree or Further qualification in a biological subject
§ Experience with Crispr cas technology, recombineering or Gateway (Gibson assembly) Cloning, qPCR, ddPCR or other relevant advanced techniques.
§ Experience of supervising and/ or training others
· Familiarity with the Animal (Scientific Procedures) Act 1986 and past or present holder of a personal licence
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